Seeing is
Discovering
- Long-read Sequencing
- Spatial Multiomics
- Single-cell Resolution
See What You’ve Been Missing
Long-Read Sequencing
Short-read sequencing often overlooks large portions of the human genome and genomes of other complex organisms, including high-GC regions, rearrangements, duplications, and inversions, which are “dark regions” of the genome. Long-read sequencing offered a solution that enabled the completion of the first comprehensive human genome – telomere-to-telomere – and many other advancements in genome science.
Long-Read Sequencing Benefits
Uncover previously unknown or difficult-to-map genes or regions of the genome (i.e., dark regions).
Phasing - identify co-inherited alleles, resolve haplotypes, and phase de novo variants.
Better, more complete de novo assembly and genome finishing.
Discover a broader range of variations, including copy-number variants (CNVs), structural variants (SVs), and medium-to-long indels.
Understanding only gene and protein expression levels in tissues or single cells doesn’t offer a complete view. Spatial transcriptomics and proteomics reveal expression levels in spatial context—tissue architecture, cell-cell interactions, microenvironments, and cellular diversity—from whole transcriptomes to single-cell resolution.
Spatial Multiomics Benefits
Resolve mRNA transcripts within intact tissue while maintaining positional information within and between cells.
Unravel new and rare cell types, gene expression heterogeneity in a cell population, and patterns of cell fate choices.
High-plex panels up to a whole transcriptome combined with quantification of transcript abundance.
Spatial Multiomics
Spatial Multiomics
Understanding only gene and protein expression levels in tissues or single cells doesn’t offer a complete view. Spatial transcriptomics and proteomics reveal expression levels in spatial context—tissue architecture, cell-cell interactions, microenvironments, and cellular diversity—from whole transcriptomes to single-cell resolution.
Spatial Multiomics Benefits
Resolve mRNA transcripts within intact tissue while maintaining positional information within and between cells.
Unravel new and rare cell types, gene expression heterogeneity in a cell population, and patterns of cell fate choices.
High-plex panels up to a whole transcriptome combined with quantification of transcript abundance.
See The Results
Publications and Announcements
October 3, 2023
A*STAR’s Genome Institute of Singapore, NanoString and Next Level Genomics Establish Joint Lab
July 11, 2023
Next Level Genomics Pte Ltd Installs First PacBio Revio Sequencer in Singapore
June 30, 2023
Next Level Genomics Pte Ltd launches dedicated PacBio long-read NGS sequencing services in Singapore
October 3, 2023
A*STAR’s Genome Institute of Singapore, NanoString and Next Level Genomics Establish Joint Lab
July 11, 2023
Next Level Genomics Pte Ltd Installs First PacBio Revio Sequencer in Singapore
June 30, 2023
Next Level Genomics Pte Ltd launches dedicated PacBio long-read NGS sequencing services in Singapore
See The Results
Publications and Announcements
April 17, 2024
Next Level Genomics Adds Oxford Nanopore’s PromethION-24 Long-Read Sequencing Services in Singapore
October 3, 2023
A*STAR’s Genome Institute of Singapore, NanoString and Next Level Genomics Establish Joint Lab
July 11, 2023
Next Level Genomics Pte Ltd Installs First PacBio Revio Sequencer in Singapore
June 30, 2023
Next Level Genomics launches dedicated PacBio long-read NGS sequencing services in Singapore.
October 3, 2023
A*STAR’s Genome Institute of Singapore, NanoString and Next Level Genomics Establish Joint Lab
July 11, 2023
Next Level Genomics Pte Ltd Installs First PacBio Revio Sequencer in Singapore
June 30, 2023
Next Level Genomics Pte Ltd launches dedicated PacBio long-read NGS sequencing services in Singapore
March 15, 2024
Gapless assembly of complete human and plant chromosomes using only nanopore sequencing
March 8, 2024
Spatially-resolved transcriptomics reveal macrophage heterogeneity and prognostic significance in diffuse large B-cell lymphoma
October 26, 2023
Spatiotemporal genomic profiling of intestinal metaplasia reveals clonal dynamics of gastric cancer progression
October 3, 2023
A*STAR’s Genome Institute of Singapore, NanoString and Next Level Genomics Establish Joint Lab
July 11, 2023
Next Level Genomics Pte Ltd Installs First PacBio Revio Sequencer in Singapore
June 30, 2023
Next Level Genomics Pte Ltd launches dedicated PacBio long-read NGS sequencing services in Singapore
See How We Work
Your Own Singapore NGS & Spatial Lab At Your Disposal
Next Level Genomics is a dedicated long-read sequencing and spatial genomics center with experienced and trained scientists in genomics and bioinformatics.
Our lab is available to everyone looking to bring the power of long-read and spatial technology to their work—and do it with a team of experts behind you. We’re here to serve you and ensure you’re getting what you want and need to get you to publication faster, more successfully with grants, and equipped with the data you need to innovate and discover.
Contact YOUR NGS & Spatial Lab at Next Level Genomics to join the long-read and spatial revolution and put us to work for you.
See How We Work
Your Own Singapore NGS & Spatial Lab At Your Disposal
Next Level Genomics is a dedicated long-read sequencing and spatial genomics centre with experienced and trained scientists in genomics and bioinformatics.
Our lab is available to everyone looking to bring the power of long-read and spatial technology to their work – and do it with a team of experts behind you.We’re here to serve you and make sure you’re getting what you want and need to get you to publication faster, more success with grants and equipped with the data you need to innovate and discovery.
Contact YOUR NGS & Spatial lab at Next Level Genomics to join the long-read and spatial revolution and put us to work for you.
See How We Work
Your Own Singapore NGS & Spatial Lab At Your Disposal
Next Level Genomics is a dedicated long-read sequencing and spatial genomics center with experienced and trained scientists in genomics and bioinformatics.
Our lab is available to everyone looking to bring the power of long-read and spatial technology to their work—and do it with a team of experts behind you. We’re here to serve you and ensure you’re getting what you want and need to get you to publication faster, more successfully with grants, and equipped with the data you need to innovate and discover.
Contact YOUR NGS & Spatial Lab at Next Level Genomics to join the long-read and spatial revolution and put us to work for you.
Our Lab
Next Level Genomics aims to offer labs personalized NGS facilities, hoping researchers see it as their own, utilizing NLG’s expertise for efficient DNA and RNA sequencing, accelerating research outcomes. The equipment is ours, but the ideas belong to you.
Tailored Solutions
By Scientists, For Scientists. Next Level Genomics, driven by scientists and seasoned leadership, collaborates closely with labs. Customized solutions, from sample handling to data analysis, efficiently accelerate unique projects toward their goals.
Bioinformatics Support
Data’s value lies in analysis and interpretation. While standard genomics workflows offer generic reports, innovative labs require flexible, advanced solutions. NLG ensures customized bioinformatic support, making complex data accessible and user-friendly from project inception.
Our Lab
Next Level Genomics aims to offer labs personalized NGS facilities, hoping researchers see it as their own, utilizing NLG’s expertise for efficient DNA and RNA sequencing, accelerating research outcomes. The equipment is ours, but the ideas belong to you.
Tailored Solutions
By Scientists, For Scientists. Next Level Genomics, driven by scientists and seasoned leadership, collaborates closely with labs. Customized solutions, from sample handling to data analysis, efficiently accelerate unique projects toward their goals.
Bioinformatics Support
Data’s value lies in analysis and interpretation. While standard genomics workflows offer generic reports, innovative labs require flexible, advanced solutions. NLG ensures customized bioinformatic support, making complex data accessible and user-friendly from project inception.
Frequently Asked Questions
Where is your lab located and where will sequencing and spatial work be performed?
Our labs are located in Singapore – both in Biopolis. Our labs are fully equipped for processing of samples to be performed in Singapore. The NLG long-read sequencing NGS lab is in the Synapse building and the spatial genomics lab is a joint lab via a research collaboration with GIS and Nanostring. All sample processing, sequencing and spatial work is done in Singapore.
What types of samples do you accept and how much is required?
For our work, we can accept primary samples—such as blood, tissues, microbiome samples, and FFPE blocks. We can extract DNA and RNA and slice FFPE blocks into slides in our labs. We also accept extracted DNA, RNA, or histopathology slides. The amount needed depends on the project—for whole genome sequencing, micrograms of DNA are best. For single-cell RNA analysis, nanograms of cDNA are best. Reach out to our specialists to discuss your projects and learn how to best proceed with samples.
Which NGS system is the best to use? Why is long-read better than short read?
No single NGS platform is perfect. Each has its own strengths and weaknesses. Long-read sequencing is superior to short-read in many aspects. With long-read data, more complex variants are easily found—things like larger deletions, insertions, rearrangements, and copy number variants—which are often missed with short-read data. In addition, long reads allow for phasing—or determining which variants are on the same copy in the genome—maternal or paternal, and therefore inheritance or transmission of these variants can be studied. When using long-read for RNA, complete isoforms of mRNA are often sequenced completely, meaning novel splicing events are discovered that might be missed with short-read sequencing.
Is data quality and integrity an issue with long-read sequencing?
No system has 100% perfect-quality scores. The latest advances in long-read sequencing, such as PacBio’s Hifi circular consensus sequencing system and Oxford Nanopore’s latest software and chemistry, have improved quality to match or beat that of short-read sequencing. Quality is a measure of the probability that a base is not the correct base found in the source DNA. One way to improve the quality and accuracy of base calling is to perform “deeper” sequencing, or sequence each strand multiple times—sometimes 20, 30, or 60 times for each base. Random errors are then removed. In the case of long-read sequencing, since many protocols utilize direct, native DNA in the sequencing without any amplification, errors are minimal since the DNA polymerase in the amplification can introduce errors in short-read protocols and lead to false variants.
Contact Us
Our representatives are available to provide additional information, answer your queries, and guide you through the next steps.
Contact Us
Our representatives are available to provide additional information, answer your queries, and guide you through the next steps.
