Long-Read Sequencing Delivers
More Data, More Insights
Long-Read Sequencing Delivers More Data, More Insights
Long-read sequencing provides enhanced structural variant detection, true variant phasing, full-length transcript analysis, and high-resolution epigenetic characterization. Generate high-quality data with reads from a few thousand bases to up to 100,000 bases in a single read. Long-read sequencing reveals regions of the genome that are difficult or impossible to sequence using short reads. Plus, long-read sequencing contains built-in DNA modification information, such as methylation—no need for additional sequencing. See what you’ve been unable to see in the past with long-read sequencing.
Long-read sequencing provides enhanced structural variant detection, true variant phasing, full-length transcript analysis, and high-resolution epigenetic characterization. Generate high-quality data with reads from a few thousand bases to up to 100,000 bases in a single read. Long-read sequencing reveals regions of the genome that are difficult or impossible to sequence using short reads. Plus, long-read sequencing contains built-in DNA modification information, such as methylation – no need for additional sequencing. See what you’ve been unable to see in the past with long-read sequencing.
The first step in harnessing the power of long-read sequencing is obtaining intact and clean high molecular weight (HMW) DNA and full-length, intact RNA for your studies.
Next Level Genomics provides DNA and RNA extraction services geared toward long-read sequencing, allowing you to take full advantage of the power of LRS—quicker and easier. Our experts have worked on a wide range of sample types, including blood, animal tissues, cells, environmental samples, and more.
Let Next Level Genomics get you started on the right foot with our HMW DNA and ultrapure RNA extraction services.
The first step in harnessing the power of long-read sequencing is obtaining intact and clean high molecular weight (HMW) DNA and full-length, intact RNA for your studies.
Next Level Genomics provides DNA and RNA extraction services geared toward long-read sequencing, allowing you to take full advantage of the power of LRS – quicker and easier. Our experts have worked on a wide range of sample types including blood, animal tissues, cells, environmental samples and more.
Let Next Level Genomics get you started on the right foot with our HMW DNA and Ultrapure RNA extraction services.
Both PacBio systems generate some of the highest quality reads – HiFi Reads – through the generation of Circular Consensus Sequences (CCS) – with read lengths up to 40kB
Revio | Machine Type | Sequel-Ile |
|---|---|---|
25M SMRT Cell – up to 90Gb data | Technical Details | 8M SMRT Cell – up to 30Gb data
|
Up to 4 cells – run in-parallel | Independent Stages | Adaptive loading – maximize output
|
Over 1100 WGS per year | Capability | Amplicons to WGS
|
Oxford Nanopore Technologies sequences directly from native DNA or native RNA and detects several base modifications for built-in epigenetic analysis and complete RNA isoform information.
Read lengths from 20 bp to ultra-long reads (ULR) possible (N50 > 50kb)
Utilized to generate the fastest human WGS on record (*Stanford University, Dr. Euan Ashley, 5 hrs)
Machine Type | PromethION-24 |
|---|---|
Technical Details | Real-time data – terabase scale |
Independent Stages | Up to 24 flow cells – like having 24 sequencers |
Capability | Over 2400 flow cells per year |
We are experts in the extraction of high-molecular-weight (HMW) DNA and intact RNA.
Determine the purity and quantity of your samples using specialized equipment for long-read analysis.
We can amplify a barcoded, complete, full-length 165 rRNA gene from your microbial samples, ready for long-read sequencing.
Sequence complex genomic regions, including repetitive sequences, structural variations, and challenging genomic areas.
Detect large and complex structural variations (SVs) in the genome, such as deletions, insertions, duplications, inversions, and translocations.
Discover causative variants of rare, undiagnosed conditions.
Find novel variants in diseases or population cohorts.
Perform disease diagnosis and genetic stratification in disease research or clinical trials.
Contribute to translational medicine and discoveries of disease and drug targets, which make it possible to develop new
treatment strategies.
Methylation information is built-in; no additional sequencing is needed.
Simplified haplotype phasing of methylated bases using long-reads.
Capability to interrogate imprinting disorders and methylation abnormalities associated with tandem repeats.
Microbial epigenetics: detecting genome-wide m6A and m4C R-M system motifs.
Twist Alliance Dark Genes panel for PacBio, a comprehensive 22 Mb panel of 389 medically relevant dark genes, including CYP2D6, GBA, SMN1/2, and PMS2.
Adaptive sampling with Oxford Nanopore allows for region-specific, targeted sequencing.
Find genes missed by short-read NGS that might typically require several platforms to adequately capture, allowing for assay consolidation and cost-effectiveness.
A comprehensive 49-gene PGx panel from Twist and Pac-Bio Community Alliance for long-read sequencing that includes all 20 current genes with CPIC guidelines, as well as FDA PGx genes and genes of research interest research interest.
Targeted haplotyping in pharmacogenomics using adaptive sampling using Oxford Nanopore from DNA or RNA.
Sequence only the genomic regions you care about—easily and cost-effectively at scale.
Adaptive sampling allows for targeted sequencing without capture—both DNA and RNA adaptive sampling.
Hybrid capture and amplicon workflows are also available to suit your needs.
Pre-designed panels or custom panels are available.
Build reference-quality, haplotype-resolved genomes and pangenomes to better understand individuals, populations, and species.
Impute traits of interest to SNPs, structural variants, and complex genotypes.
Capture genomic variants on a genome-wide scale for outbreds, inbreds, and populations.
Long-read sequencing has revolutionized the study of pan-genomes beyond microbial genomes.
Capture the entire set of genes within a species, consisting of a core genome—containing sequences shared between all individuals of the species—and the ‘dispensable’ genome.
Pan-genome analysis allows for the discovery of variants that are missing from the reference genome but may be associated with specific phenotypes.
Nanopore direct single-molecule RNA sequencing is a highly parallel, real-time, single-molecule method without prior conversion of RNA to cDNA.
Low input amounts and streamlined workflows enable highly sensitive gene expression analysis.
Full-length transcripts of all isoforms delivered by nanopore sequencing allow identification of splice variants and gene fusions.
Discover alternative splicing events, including alternative start sites, end sites, intron retention, and exon-skipping events.
Find novel gene fusions and identify allele-specific isoforms.
Detect differentially expressed isoforms down to single-cell resolution.
From input single-cell cDNA, utilizing novel concatenation methods, generate a 16-fold throughput increase compared to regular single-cell Iso-Seq libraries with new kits from PacBio.
Available on both the PacBio and Oxford Nanopore platforms.
Compatible with cDNA generated using the 10x Chromium Next GEM Single Cell 3’ kit (v3.1) and Single Cell 5’ kit (v2), it is intended for use on a 3,000 to 10,000 cell library with 15–75 ng of cDNA as input.
Utilize the adaptive sampling function of Oxford Nanopore sequencing to selectively deplete and enrich RNAs of interest without biochemical manipulation before sequencing.
Identify previously unknown transcripts and isoforms, including those that are antisense to existing transcripts.
Depleting unwanted transcripts can enrich RNAs of interest.
Generate the highest-quality, closed-reference genomes.
Identify ever-evolving genes associated with toxicity, virulence, and antimicrobial resistance.
Resolve strains, serotypes, and plasmids to track pathogen outbreaks in humans, plants, and animals through food systems, hospitals, and communities.
Comprehensively characterize microbes to facilitate scientific breakthroughs.
Overcome misclassification at the species level using full-length 16S rRNA sequencing with long reads to resolve similarity in each 16S variable region.
The Kinnex 16S rRNA kits from PacBio increase throughput, increasing the resolution of microbial analysis.
Massive multiplexing capabilities on both the PacBio and Oxford Nanopore platforms.
Identify 6–8 full-length genes with efficient, cost-effective metagenomic profiling.
Generate hundreds of high-quality (HQ) metagenome-assembled genomes (MAGs), many of which are single-contiguous, circular MAGs.
Leverage epigenomic data to associate contigs and plasmids from closely related strains.
Determine community composition at the species or strain level with competitively priced full-length 16S sequencing.
Achieve the taxonomic and functional resolution needed to generate testable hypotheses about a microbiota’s impact on health, disease, the environment, and more.
Resolve structural variants in the microbiome and expand the detection scope of genetic variations, enabling the profiling of strain-level variation.
We are experts in extraction of high molecular weight (HMW) DNA and intact RNA
Determine the purity and quantity of your samples using specialized equipment for long-read analysis
We can amplify barcoded complete, full-length 165 rRNA gene from your microbial samples ready for long-read sequencing
The best place to start is to contact our NGS specialists to discuss your project goals and needs. Even prior to beginning a project, we can advise on ways to make the best use of long-read sequencing – from extraction method to multiplexing to sequencing strategies – we are there to help you get the most out of your long-read project in the most efficient and economical manner. We can pass along publications, tech notes and other materials related to your project to demonstrate the power of long-read sequencing and benefits you’ll obtain.
For samples within Singapore, Next Level Genomics can arrange for sample collection from your lab at your convenience. Alternatively, samples can be dropped off at our labs in Biopolis. We are happy to discuss your project with you and what works best for getting your samples to our lab in the best manner. Samples from outside Singapore can be shipped directly to our labs based on your needs.
Each project is unique in sample type, sample number, and output requirements. For whole genome sequencing of DNA, libraries can often be prepared within 1-2 weeks of the quality clearance of the sample. Long-read sequencing generally takes 36–40 hours. With the Revio system, multiple samples can run in parallel, shortening the run time for a project. Spatial slides can be processed in 1-2 weeks, and spatial imaging or profiling can last from hours to days, depending on the project. Contact our lab specialists to discuss your project details and learn more about how we can serve you best and most efficiently.
Next Level Genomics provides bioinformatics and data analysis services utilizing a combination of tools provided by equipment manufacturers such as Smart Link from PacBio and AtoMx from Nanostring, as well as custom bioinformatics to suit your needs. Data can be delivered to you via the cloud, FTP, or physical hard drive. Speak with our specialists to learn how Next Level Genomics can provide complete end-to-end service, from sample extraction and handling to data generation and full analysis.
Yes, Next Level Genomics has all the equipment and experience in DNA and RNA extraction to take your primary samples and perform complete end-to-end processing. As long-read sequencing experts, we have experience in the isolation of high-molecular-weight (HMW) DNA that is perfect for both PacBio and Oxford Nanopore sequencing. Speak to our specialists to learn how we can assist in getting your project off on the right foot with optimum extraction.
Read length can vary based on your project’s needs. Microbial genomes and targeted approaches can focus on reads between 5 kb-10 kb, while whole genome analysis may need 15 kb-20 kb. With nanopore sequencing, ultra-long reads of 50kb or larger are possible. The amount of data generated can be customized to your project by utilizing multiplexing and barcodes to control the number of samples on a flowcell and the amount of data generated. Human genomes from 25X to 30X are possible on a single flow cell with both systems Next Level Genomics uses.
Yes, with UMIs and barcoded adapters, samples can be individually tagged and multiplexed to customize the amount of data generated per sample—this can often account for significant savings on the costs of long-read sequencing. In addition, by submitting multiple samples for processing at the same time, savings can be made by batching the libraries during the quality control (QC) steps.
With both long-read platforms – PacBio and Oxford Nanopore – DNA modifications are including the sequencing results – built-in. No additional treatments or sequencing is needed to obtain epigenetic information from your samples. Discuss your needs with our specialists to learn how built-in modification information can benefit your project.
Unlock the potential of your genomics projects with personalized guidance. Connect with our seasoned experts at Next Level Genomics to discuss your goals, explore possibilities, and pave the way for groundbreaking discoveries. Fill out the form to initiate a discussion and propel your research forward.
Next Level Genomics (NLG) is a cutting-edge genomics provider offering long-read sequencing (LRS) and spatial genomics capabilities in our two laboratories in Singapore, located in Biopolis.
Main Office and NGS Lab
Next Level Genomics Pte Ltd
3 Bipolis Drive, #05-19 Synapse
Singapore 138623
Spatial Biology Lab
60 Biopolis Street
#05-07 Genome
Singapore 138672
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