Long-Read Sequencing Delivers
More Data, More Insights
Spatial Genomics Puts Gene And Protein Expression Data Before Your Very Eyes
Spatial Genomics puts gene and protein expression data before your very eyes
Spatial genomics is a true game-changer when it comes to studying the complexity of biological samples. Simply knowing what genes are expressed or the level of their expression is often not enough to gain a full understanding of biological systems. With spatial genomics, you get not only the genes that are expressed and the level, but also the POSITION within a cell or tissue and how they relate to each other. Truly a 3-dimensional view of biology.
Spatial genomics starts with tissue samples – FFPE or frozen – blocks or slides. Next Level Genomics can work with you to get your samples ready for success with spatial analysis with microtome services or from your own slides. Our histopathology and spatial experts will work with you to understand your project and needs. The selection of cell morphology markers, cell typing markers, and target panels can all be customized to maximize the data output for your project. Data analysis and visualization are available, or raw data can be delivered to you for your own analysis.
Let Next Level Genomics guide you on your spatial journey and discover the depth of data and discoveries available with the latest in spatial genomics technologies.
Long-Read Sequencing has allowed for the first true completion of the human genome and so many other genomes. Long-Read Sequencing allows for previously problematic regions of DNA to be sequenced and assembled including repeats, low complexity regions and more. For RNA sequencing, never-before-seen RNA isoforms have been revealed using long-read sequencing and when utilized for single-cell RNA sequencing or microbial population analysis, unimaginable resolution can be attained to reveal more biology and greater insights.
The power of long-read sequencing is truly unveiled when starting with long DNA or RNA. The extraction of the most intact and clean high molecular weight (HMW) DNA and intact RNA is the starting point to unlocking the mysteries hidden within.
Next Level Genomics provides DNA and RNA extraction services geared toward long-read sequencing which allows you to take full advantage of the power of LRS – quicker and easier. Our experts have worked on a wide range of sample types including blood, animal tissues, cells, environmental samples and more.
Let Next Level Genomics get you off on the right start with our HMW DNA and Ultrapure RNA extraction services.
Life is three-dimensional. To truly understand the dynamics and interactions within cells, tissues, and organisms, you need to look at things in the context of their spatial order and hierarchy. This is now possible on an unprecedented scale, using the latest technologies in spatial genomics. From complete protein catalogs to whole transcriptomes, down to single-cell resolution.
The CosMx Spatial Molecular Imager from Nanostring is the highest-plex in situ analysis platform to provide spatial multiomics with formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissue samples at cellular and subcellular resolution. CosMx SMI enables rapid quantification and visualization of up to 6,000 RNA and 64 validated protein analytes. [Soon to be whole transcriptome profiling].
The GeoMx Digital Spatial Profiler (DSP) from Nanostring is a flexible, robust spatial multiomic platform for the analysis of FFPE and fresh frozen tissue sections. GeoMx is the only spatial biology platform that non-destructively profiles the expression of RNA and protein from distinct tissue compartments and cell populations with an automated and scalable workflow that integrates with standard histology staining.
nCounter provides multiplexing up to 800 gene expression targets using direct detection technology. The workflow requires just 15 minutes of hands-on time for most panels using an automated benchtop processing system – requiring no amplification or technical replicates. Rapidly accelerate your biomarker validation and biomarker development with gene expression data you can count on.
The CosMx Spatial Molecular Imager from Nanostring is the highest-plex in situ analysis platform to provide spatial multiomics with formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissue samples at cellular and subcellular resolution. CosMx SMI enables rapid quantification and visualization of up to 6,000 RNA and 64 validated protein analytes. [Soon to be whole transcriptome profiling].
Sample Image of Data
The GeoMx Digital Spatial Profiler (DSP) from Nanostring is a flexible, robust spatial multiomic platform for analysis of FFPE and fresh frozen tissue sections. GeoMx is the only spatial biology platform that non-destructively profiles expression of RNA and protein from distinct tissue compartments and cell populations with an automated and scalable workflow that integrates with standard histology staining.
Sample Image of Data
nCounter provides multiplexing up to 800 gene expression targets using direct detection technology. The workflow requires just 15 minutes hands-on time for most panels using an automated benchtop processing system – requiring no amplification or technical replicates. Rapidly accelerate your biomarker validation and biomarker development with gene expression data you can count on.
Sample Image of Data
We are experienced in FPPE-block and frozen tissue microtome slicing and slide preparation for spatial genomics analysis. H&E staining and other staining services are also available.
We are experienced in FPPE-block and frozen tissue microtome slicing and slide preparation for spatial genomics analysis. H&E staining and other staining services are also available.
6000 RNA targets – the most significant genes across every biological pathway + over 400 ligand-receptor pairs.
A nuclear stain plus four protein markers for cell segmentation.
Customize with up to 200 additional RNA targets.
Expression of 1000 highly curated targets at subcellular resolution.
Customize with up to 50 of your targets.
Up to five CosMx Segmentation Markers.
Expression of 100 transcripts covering immune cells, their activities, and aspects of tumor biology relevant to IO therapies.
Customize with up to ten of your own targets.
100-plex format offers faster turnaround and lower overall cost than high-plex panels.
Robust cell typing, cell-cell interaction analysis, and more in the mouse brain and other neuronal tissues.
1000 highly curated targets at subcellular resolution and customize with up to 50 of your own targets.
Identification of 42 distinct cell types in the mouse brain.
Unbiased view of 18,000+ protein-coding genes – human or mouse.
Robust and sensitive performance on FFPE or Fresh Frozen samples.
Compatible with RNAscope® and antibody morphology markers.
For use with next-generation sequencer (NGS) readouts and compatible with DSP Data Center.
Comprehensive profiling of tumor biology, the tumor microenvironment, and the immune response.
RNA expression of over 1,800 genes.
Measure clinically-derived gene sets, including the 18-gene Tumor Inflammation Signature (TIS), known to be associated with response to PD-1/PD-L1 inhibitor pathway blockade, and the 50-gene Prediction Analysis of Microarray 50 Signature (PAM50), known to be associated with breast cancer metastasis.
Non-destructively profile over 570 proteins in different tissue compartments, such as the tumor, microenvironment, and immune infiltrate.
The highest-plex spatial proteomics panel is available for FFPE and fresh frozen tissue sections.
Customize with up to 40 additional protein targets of your choice.
Be the first to discover new multiomic biomarkers.
PanCancer IO 360 – 750 cancer-related genes.
PanCancer Immune Profiling – 770 immune profiling genes.
Tumor Signaling 360 – 760 genes covering the core pathways and processes of the tumor.
nCounter® Gene Therapy Optimization Panel – 800 genes covering processes known to impact gene therapy development and manufacturing.
PanCancer Pathways – 770 genes for essential cancer pathways.
and many more.
The nCounter® platform offers the flexibility to tailor assay content to meet individual project needs with a triad of custom solutions.
Custom CodeSets, Elements™ TagSets, PlexSet™ Reagents, and Plus Content.
Spatial genomics provides the most in-depth picture of the biology of a sample – from the spatial context in which cells and interactions are taking place to the extra-cellular microenvironment to morphological changes. Spatial is orders of magnitude more information than single-cell RNAseq or other methods of examining cell signaling and response. If you would like to know not just who the players are in your sample but where they are and how they interact, spatial genomics is for you.
Yes, all assays on the Nanostring systems allow for customization with the addition of probes for your specific genes of interest. And any antibodies against your favorite protein target can be labeled and utilized for spatial analysis on these systems. For the nCounter, the whole custom panel design and validation are available. Contact our spatial specialist for information on gene lists and how to customize assays for your needs.
Spatial analysis involves slide preparation, processing, and scanning on the instruments. Slide preparation can be done by Next Level Genomics, or you can pass your own slides for processing. Slide processing takes 1-2 days, and they are ready to go onto the instrument. On the GeoMx DSP platform, scanning takes just a few hours, and regions of interest are selected and captured for downstream NGS analysis. NGS sequencing can take a few weeks before the data is available for final analysis. On the CosMx platform, the slides are scanned and fields of view are selected for analysis, and over 4–8 days, the data is collected and captured in real-time. Upon completion of the CosMx run, the data is available on the cloud-based AtoMx platform for immediate analysis.
The Nanostring Spatial ecosystem includes the cloud-based AtoMx bioinformatics system for the analysis of data. Pipelines consisting of transcriptomic analysis, clustering, PCA/UMAP, neighborhood analysis, cell typing, and cell-cell interactions are all available on the AtoMx system. In addition, raw data is available for download and offline analysis by the Next Level Genomics team or your own bioinformatics team.
Yes, assays include cell segmentation protein markers and cell typing markers, as well as the capability to explore additional protein targets with your RNA panel. Nanostring systems are non-destructive in their method of data capture, which allows for the analysis of both RNA and protein on a slide.
Nanostring GeoMx and CosMx systems perform best when using slides that bind tightly to FFPE slides, such as Leica Bond slides. Standard FFPE blocks can be used for the nanostring systems. No new tissue samples or biopsies are needed. Contact our spatial specialist to learn more about slides and processing and how to maximize your data using Nanostring spatial systems.
Unlock the potential of your genomics projects with personalized guidance. Connect with our seasoned experts at Next Level Genomics to discuss your goals, explore possibilities, and pave the way for groundbreaking discoveries. Fill out the form to initiate a discussion and propel your research forward.
Next Level Genomics (NLG) is a cutting-edge genomics provider offering long-read sequencing (LRS) and spatial genomics capabilities in our two laboratories in Singapore, located in Biopolis.
Main Office and NGS Lab
Next Level Genomics Pte Ltd
3 Bipolis Drive, #05-19 Synapse
Singapore 138623
Spatial Biology Lab
60 Biopolis Street
#05-07 Genome
Singapore 138672
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