Seeing is
Discovering

See What You’ve Been Missing

Long-Read Sequencing

Short-read sequencing often overlooks large portions of the human genome and genomes of other complex organisms, including high-GC regions, rearrangements, duplications, and inversions, which are “dark regions” of the genome. Long-read sequencing offered a solution that enabled the completion of the first comprehensive human genome – telomer-to-telomere – and many other advancements in genome science.

Long-Read Sequencing Benefits

Uncover previously unknown or difficult-to-map genes or regions of the genome (ie. dark regions)

Phasing - identify co-inherited alleles, resolve haplotypes, and phase de novo variants

Better, more complete de novo assembly and genome finishing

Discover a broader range of variations such as copy-number variants (CNVs), structural variants (SVs) and medium to long Indels

Understanding only gene and protein expression levels in tissues or single cells doesn’t offer a complete view. Spatial transcriptomics and proteomics reveal expression levels in spatial context…tissue architecture, cell-cell interactions, microenvironments, and cellular diversity, from whole transcriptomes down to single-cell resolution.

Spatial Multiomics Benefits

Resolve mRNA transcripts within intact tissue while maintaining positional information within and between cells

Unravel new and rare cell types, gene expression heterogeneity in a cell population, and patterns of cell fate choices

High-plex panels up to a whole transcriptome combined with quantification of transcript abundance

Spatial Multiomics

Spatial Multiomics

Understanding only gene and protein expression levels in tissues or single cells doesn’t offer a complete view. Spatial transcriptomics and proteomics reveal expression levels in spatial context…tissue architecture, cell-cell interactions, microenvironments, and cellular diversity, from whole transcriptomes down to single-cell resolution.

Spatial Multiomics Benefits

Resolve mRNA transcripts within intact tissue while maintaining positional information within and between cells

Unravel new and rare cell types, gene expression heterogeneity in a cell population, and patterns of cell fate choices

High-plex panels up to a whole transcriptome combined with quantification of transcript abundance

See The Results

Publications and Announcements

See The Results

Publications and Announcements

See How We Work

Your Own Singapore NGS & Spatial Lab At Your Disposal

Next Level Genomics is a dedicated long-read sequencing and spatial genomics centre with experienced and trained scientists in genomics and bioinformatics.

Our lab is available to everyone looking to bring the power of long-read and spatial technology to their work – and do it with a team of experts behind you. We’re here to serve you and make sure you’re getting what you want and need to get you to publication faster, more success with grants and equipped with the data you need to innovate and discovery.

Contact YOUR NGS & Spatial lab at Next Level Genomics to join the long-read and spatial revolution and put us to work for you.

See How We Work

Your Own Singapore NGS & Spatial Lab At Your Disposal

Next Level Genomics is a dedicated long-read sequencing and spatial genomics centre with experienced and trained scientists in genomics and bioinformatics.

Our lab is available to everyone looking to bring the power of long-read and spatial technology to their work – and do it with a team of experts behind you.We’re here to serve you and make sure you’re getting what you want and need to get you to publication faster, more success with grants and equipped with the data you need to innovate and discovery.

Contact YOUR NGS & Spatial lab at Next Level Genomics to join the long-read and spatial revolution and put us to work for you.

See How We Work

Your Own Singapore NGS & Spatial Lab At Your Disposal

Next Level Genomics is a dedicated long-read sequencing and spatial genomics centre with experienced and trained scientists in genomics and bioinformatics.

Our lab is available to everyone looking to bring the power of long-read and spatial technology to their work – and do it with a team of experts behind you.We’re here to serve you and make sure you’re getting what you want and need to get you to publication faster, more success with grants and equipped with the data you need to innovate and discovery.

Contact YOUR NGS & Spatial lab at Next Level Genomics to join the long-read and spatial revolution and put us to work for you.

Our Lab

Next Level Genomics aims to offer labs personalized NGS facilities, hoping researchers see it as their own, utilizing NLG’s expertise for efficient DNA and RNA sequencing, accelerating research outcomes. The equipment is ours, but the ideas belong to you.

Tailored Solutions

By Scientists, For Scientists. Next Level Genomics, driven by scientists and seasoned leadership, collaborates closely with labs. Customized solutions, from sample handling to data analysis, accelerate unique projects towards their goals efficiently.

Bioinformatics Support

Data’s value lies in analysis and interpretation. While standard genomics workflows offer generic reports, innovative labs require flexible and advanced solutions. NLG ensures customized bioinformatic support, making complex data accessible and user-friendly from project inception.

Our Lab

Next Level Genomics aims to offer labs personalized NGS facilities, hoping researchers see it as their own, utilizing NLG’s expertise for efficient DNA and RNA sequencing, accelerating research outcomes. The equipment is ours, but the ideas belong to you.

Tailored Solutions

By Scientists, For Scientists. Next Level Genomics, driven by scientists and seasoned leadership, collaborates closely with labs. Customized solutions, from sample handling to data analysis, accelerate unique projects towards their goals efficiently.

Bioinformatics Support

Data’s value lies in analysis and interpretation. While standard genomics workflows offer generic reports, innovative labs require flexible and advanced solutions. NLG ensures customized bioinformatic support, making complex data accessible and user-friendly from project inception.

Frequently Asked Questions

Where is your lab located and where will sequencing and spatial work be performed?

Our labs are located in Singapore – both in Biopolis. Our labs are fully equipped for processing of samples to be performed in Singapore. The NLG long-read sequencing NGS lab is in the Synapse building and the spatial genomics lab is a joint lab via a research collaboration with GIS and Nanostring. All sample processing, sequencing and spatial work is done in Singapore.

For all of our work, we can accept primary samples – such as blood, tissues, microbiome samples and FFPE blocks. We can extract DNA and RNA and also slice FFPE blocks into slides in our labs. We also accept extracted DNA or RNA or histopathology slides. The amount needed is dependent on the project – for whole genome sequencing, micrograms of DNA is best. For single-cell RNA analysis, nanograms of cDNA. Reach out to our specialists to discuss your projects to learn how to best proceed with samples.

No single NGS platform is perfect. Each has their own strengths and weaknesses. Long-read sequencing is superior to short-read in many aspects. With long-read data, more complex variants are easily found – things like larger deletions, insertions, rearrangements and copy number variants – which are often missed with short read data. In addition, long read allows for phasing – or determining which variants are on the same copy in the genome – maternal or paternal – and therefore inheritance or transmission of these variants can be studied. When using long-read for RNA, complete isoforms of mRNA are often sequenced completely, meaning novel splicing events are discovered that might be missed with short-read sequencing.

No system has 100% perfect quality scores. The latest advances in long-read sequencing, such as PacBio’s Hifi circular consensus sequencing system and Oxford Nanopore’s latest software and chemistry have improved quality to match or beat that of short-read sequencing. Quality is a measure of the probability that a base called is not the correct base found in the source DNA. One way to improve of the quality and accuracy of base calling is to perform “deeper” sequencing – or sequence each strand multiple times – sometimes 20, 30 or 60 times for each base. Random errors are then removed. In the case of long-read sequencing, since many protocols utilize direct, native DNA in the sequencing – without any amplification – errors are minimal since the DNA polymerase in the amplification can introduce errors in short-read protocols and lead to false variants.

Contact Us

Our representatives are available to provide additional information, answer your queries, and guide you through the next steps.

Contact Us

Our representatives are available to provide additional information, answer your queries, and guide you through the next steps.